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pA11990.gb
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pA11990.gb
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LOCUS pA11990 6705 bp DNA circular 06-FEB-2019
FEATURES Location/Qualifiers
promoter 1..19
/label="T7 prom"
misc_feature 19..46
/label="lacO reg"
misc_feature 53..127
/label="RiboJ10"
primer_bind 89..106
/label="oIG00007"
primer complement(94..127)
/label="pARZ4_backbone_new_REV"
primer_bind complement(94..122)
/label="oIG00006"
RBS 128..169
/label="RiboJ10-RBSmax-F13557"
CDS 170..1315
/label="F13557"
misc_feature 170..1315
/label="insert synthon"
primer_bind 424..469
/label="oIG00005"
primer_bind complement(424..453)
/label="oIG00008"
primer 1316..1344
/label="pARZ4_backbone_new_FOR"
terminator 1316..1516
/label="DT10"
plasmid 1316..127
/label="backbone plasmid"
misc_feature 1517..1556
/label="INS230"
misc_structure 1557..1562
/label="linker"
misc_feature 1563..1580
/label="6X His tag"
terminator 1591..1719
/label="T7 term"
rep_origin 1730..2174
/label="M13 origin"
rep_origin 1747..2053
/label="f1 origin"
CDS complement(2278..3093)
/label="kan2 marker"
rep_origin 3139..3821
/label="colE1 origin"
terminator 3195..3237
/label="terminator"
rep_origin 3199..3818
/label="pBR322 origin"
terminator 3688..3728
/label="putative terminator"
terminator 3796..3863
/label="putative terminator(1)"
terminator 4419..4458
/label="putative terminator(2)"
CDS 4456..4824
/label="uncharacterized protein"
CDS complement(4821..5177)
/label="tet"
CDS complement(5233..6315)
/label="lacI"
5'UTR complement(6316..6343)
/label="lacI 5' UTR"
misc_feature complement(6407..6670)
/label="tet(1)"
ORIGIN
1 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAGCGCTCA
61 ACGGGTGTGC TTCCCGTTCT GATGAGTCCG TGAGGACGAA AGCGCCTCTA CAAATAATTT
121 TGTTTAAAGT AGAGCTCGCG TTTTTTAGCG ATAAGGAGGT TTTTTTTTTA TGCGTGTAGG
181 AATCCCGACT GAAACCAAAA ACAATGAGTT CCGTGTTGCG ATTACCCCAG CGGGCGTGGC
241 GGAACTGACC CGTCGTGGTC ACGAAGTGCT GATTCAGGCG GGTGCGGGCG AAGGTTCTGC
301 GATTACCGAT GCAGATTTCA AAGCGGCAGG TGCCCAGTTG GTGGGCACCG CTGACCAGGT
361 GTGGGCGGAT GCCGACCTGC TGTTGAAAGT CAAAGAACCG ATTGCCGCAG AGTATGGTCG
421 TCTGCGTCAC GGTCAGATTC TGTTTACCTT TCTCCACCTG GCGGCAAGCC GTGCCTGTAC
481 CGATGCCCTG CTGGATAGCG GCACGACCAG CATTGCCTAT GAAACCGTTC AGACCGCAGA
541 TGGTGCCCTG CCGTTACTGG CACCGATGAG TGAAGTGGCG GGTCGACTTG CGGCACAAGT
601 TGGTGCGTAT CACCTGATGC GTACCCAGGG CGGTCGTGGC GTGCTGATGG GCGGTGTTCC
661 AGGAGTGGAA CCAGCGGATG TGGTTGTGAT TGGTGCTGGC ACCGCAGGCT ATAATGCGGC
721 ACGCATTGCC AATGGTATGG GTGCGACGGT GACCGTTCTG GACATCAACA TTGATAAACT
781 GCGTCAACTG GATGCCGAGT TTTGCGGTCG TATTCACACC CGTTATTCCA GTGCGTATGA
841 ACTGGAAGGT GCGGTGAAGC GTGCGGATTT AGTTATTGGT GCCGTGCTGG TGCCAGGTGC
901 GAAAGCCCCG AAACTGGTGA GCAACAGCCT GGTTGCCCAC ATGAAACCAG GTGCCGTTCT
961 GGTGGACATT GCCATTGACC AGGGCGGTTG CTTTGAAGGC AGCCGCCCGA CCACCTATGA
1021 CCATCCGACC TTCGCCGTTC ACGATACCCT GTTTTATTGC GTGGCGAATA TGCCAGCGTC
1081 GGTGCCGAAA ACCAGCACCT ATGCCCTGAC CAACGCCACG ATGCCGTATG TTCTGGAACT
1141 GGCGGACCAC GGCTGGCGTG CGGCGTGTCG AAGCAATCCA GCACTGGCGA AAGGTCTGAG
1201 CACCCACGAA GGTGCTCTGC TGAGCGAACG GGTTGCAACC GATTTAGGCG TGCCGTTTAC
1261 CGAACCAGCG TCTGTTCTGG CGGGCAGCGG CAGTCACCAT CATCATCACC ATTAATTCAG
1321 CCAAAAAACT TAAGACCGCC GGTCTTGTCC ACTACCTTGC AGTAATGCGG TGGACAGGAT
1381 CGGCGGTTTT CTTTTCTCTT CTCAATACAA ATGAAAGTAC ATAGAAATTA CTCGGTACCA
1441 AATTCCAGAA AAGAGGCCTC CCGAAAGGGG GGCCTTTTTT CGTTTTGGTC CTCATAGGCA
1501 ATACGATCGC ATGTCCGTTC TACCCTGGAC TTACAGTTGT CGGCTGAAAG CGAAACCTCG
1561 AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT
1621 TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT
1681 TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATG GCGAATGGGA CGCGCCCTGT
1741 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
1801 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
1861 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
1921 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
1981 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
2041 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
2101 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
2161 AACAAAATAT TAACGTTTAC AATTTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC
2221 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAA TTAATTCTTA
2281 GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC
2341 ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG
2401 GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT
2461 TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA
2521 ATCCGGTGAG AATGGCAAAA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC
2581 ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC
2641 CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG
2701 CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC
2761 TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC
2821 AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG
2881 TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA
2941 CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT GCCCGACATT
3001 ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT
3061 AGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA
3121 AGCAGACAGT TTTATTGTTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG
3181 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
3241 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
3301 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
3361 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
3421 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
3481 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
3541 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
3601 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
3661 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
3721 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
3781 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
3841 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
3901 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
3961 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT
4021 GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
4081 GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC
4141 CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA
4201 CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA
4261 CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT
4321 GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG
4381 ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA
4441 GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA
4501 CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG
4561 GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT
4621 ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA
4681 ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC
4741 ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG
4801 CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC
4861 AACGACAGGA GCACGATCAT GCGCACCCGT GGGGCCGCCA TGCCGGCGAT AATGGCCTGC
4921 TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG
4981 ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG
5041 CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA
5101 GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG
5161 AAGGCTCTCA AGGGCATCGG TCGAGATCCC GGTGCCTAAT GAGTGAGCTA ACTTACATTA
5221 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
5281 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCCAGGG TGGTTTTTCT
5341 TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC GCCTGGCCCT GAGAGAGTTG
5401 CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA TCCTGTTTGA TGGTGGTTAA
5461 CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT CCCACTACCG AGATGTCCGC
5521 ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG CCCAGCGCCA TCTGATCGTT
5581 GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC ATTTGCATGG TTTGTTGAAA
5641 ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC GGCTGAATTT GATTGCGAGT
5701 GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG ACAGAACTTA ATGGGCCCGC
5761 TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC TCCACGCCCA GTCGCGTACC
5821 GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG TCAGAGACAT CAAGAAATAA
5881 CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA TCCTGGTCAT CCAGCGGATA
5941 GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG TGCACCGCCG CTTTACAGGC
6001 TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG GCACCCAGTT GATCGGCGCG
6061 AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG GCCAGACTGG AGGTGGCAAC
6121 GCCAATCAGC AACGACTGTT TGCCCGCCAG TTGTTGTGCC ACGCGGTTGG GAATGTAATT
6181 CAGCTCCGCC ATCGCCGCTT CCACTTTTTC CCGCGTTTTC GCAGAAACGT GGCTGGCCTG
6241 GTTCACCACG CGGGAAACGG TCTGATAAGA GACACCGGCA TACTCTGCGA CATCGTATAA
6301 CGTTACTGGT TTCACATTCA CCACCCTGAA TTGACTCTCT TCCGGGCGCT ATCATGCCAT
6361 ACCGCGAAAG GTTTTGCGCC ATTCGATGGT GTCCGGGATC TCGACGCTCT CCCTTATGCG
6421 ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA
6481 GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA
6541 TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG
6601 TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA
6661 TGCGTCCGGC GTAGAGGATC GAGATCGATC TCGATCCCGC GAAAT
//