diff --git a/CHANGELOG.md b/CHANGELOG.md
index 0e26a16c58b4..80d3a97e863d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,6 +8,7 @@
 
 ### Chore & Maintenance
 
+- `[jest-runtime]` [**BREAKING**] Remove long-deprecated `require.requireActual` and `require.requireMock` methods ([#9854](https://github.com/facebook/jest/pull/9854))
 - `[expect, jest-mock, pretty-format]` [**BREAKING**] Remove `build-es5` from package ([#9945](https://github.com/facebook/jest/pull/9945))
 
 ### Performance
diff --git a/packages/jest-environment/src/index.ts b/packages/jest-environment/src/index.ts
index 42ca2979c3bd..f48f1131ac39 100644
--- a/packages/jest-environment/src/index.ts
+++ b/packages/jest-environment/src/index.ts
@@ -58,11 +58,6 @@ export declare class JestEnvironment {
 
 export type Module = NodeModule;
 
-export interface LocalModuleRequire extends NodeRequire {
-  requireActual(moduleName: string): unknown;
-  requireMock(moduleName: string): unknown;
-}
-
 // TODO: Move to some separate package
 export interface Jest {
   /**
diff --git a/packages/jest-haste-map/src/lib/__tests__/dependencyExtractor.test.js b/packages/jest-haste-map/src/lib/__tests__/dependencyExtractor.test.js
index 6ce7e444fae9..d4f8ab691942 100644
--- a/packages/jest-haste-map/src/lib/__tests__/dependencyExtractor.test.js
+++ b/packages/jest-haste-map/src/lib/__tests__/dependencyExtractor.test.js
@@ -184,46 +184,6 @@ describe('dependencyExtractor', () => {
     expect(extract(code)).toEqual(new Set(['dep1', 'dep2', 'dep3']));
   });
 
-  it('should extract dependencies from `require.requireActual` calls', () => {
-    const code = `
-      // Good
-      require.requireActual('dep1');
-      const dep2 = require.requireActual(
-        "dep2",
-      );
-      if (require.requireActual(\`dep3\`).cond) {}
-      require
-        .requireActual('dep4');
-
-      // Bad
-      ${COMMENT_NO_NEG_LB} foo . require.requireActual('inv1')
-      xrequire.requireActual('inv2');
-      require.requireActualx('inv3');
-      require.requireActual('inv4', 'inv5');
-    `;
-    expect(extract(code)).toEqual(new Set(['dep1', 'dep2', 'dep3', 'dep4']));
-  });
-
-  it('should extract dependencies from `require.requireMock` calls', () => {
-    const code = `
-      // Good
-      require.requireMock('dep1');
-      const dep2 = require.requireMock(
-        "dep2",
-      );
-      if (require.requireMock(\`dep3\`).cond) {}
-      require
-        .requireMock('dep4');
-
-      // Bad
-      ${COMMENT_NO_NEG_LB} foo . require.requireMock('inv1')
-      xrequire.requireMock('inv2');
-      require.requireMockx('inv3');
-      require.requireMock('inv4', 'inv5');
-    `;
-    expect(extract(code)).toEqual(new Set(['dep1', 'dep2', 'dep3', 'dep4']));
-  });
-
   it('should extract dependencies from `jest.requireActual` calls', () => {
     const code = `
       // Good
diff --git a/packages/jest-haste-map/src/lib/dependencyExtractor.ts b/packages/jest-haste-map/src/lib/dependencyExtractor.ts
index d1aa4445ff3c..e99a513701ec 100644
--- a/packages/jest-haste-map/src/lib/dependencyExtractor.ts
+++ b/packages/jest-haste-map/src/lib/dependencyExtractor.ts
@@ -63,7 +63,6 @@ const IMPORT_OR_EXPORT_RE = createRegExp(
 const JEST_EXTENSIONS_RE = createRegExp(
   [
     ...functionCallStart(
-      'require\\s*\\.\\s*(?:requireActual|requireMock)',
       'jest\\s*\\.\\s*(?:requireActual|requireMock|genMockFromModule)',
     ),
     CAPTURE_STRING_LITERAL(1),
diff --git a/packages/jest-runtime/src/index.ts b/packages/jest-runtime/src/index.ts
index 3b7b5003d1e7..1cf4cb0c0c55 100644
--- a/packages/jest-runtime/src/index.ts
+++ b/packages/jest-runtime/src/index.ts
@@ -23,7 +23,6 @@ import type {Config, Global} from '@jest/types';
 import type {
   Jest,
   JestEnvironment,
-  LocalModuleRequire,
   Module,
   ModuleWrapper,
 } from '@jest/environment';
@@ -1314,7 +1313,7 @@ class Runtime {
   private _createRequireImplementation(
     from: InitialModule,
     options?: InternalModuleOptions,
-  ): LocalModuleRequire {
+  ): NodeRequire {
     // TODO: somehow avoid having to type the arguments - they should come from `NodeRequire/LocalModuleRequire.resolve`
     const resolve = (moduleName: string, options: ResolveOptions) =>
       this._requireResolve(from.filename, moduleName, options);
@@ -1324,13 +1323,8 @@ class Runtime {
     const moduleRequire = (options?.isInternalModule
       ? (moduleName: string) =>
           this.requireInternalModule(from.filename, moduleName)
-      : this.requireModuleOrMock.bind(
-          this,
-          from.filename,
-        )) as LocalModuleRequire;
+      : this.requireModuleOrMock.bind(this, from.filename)) as NodeRequire;
     moduleRequire.extensions = Object.create(null);
-    moduleRequire.requireActual = this.requireActual.bind(this, from.filename);
-    moduleRequire.requireMock = this.requireMock.bind(this, from.filename);
     moduleRequire.resolve = resolve;
     moduleRequire.cache = (() => {
       // TODO: consider warning somehow that this does nothing. We should support deletions, anyways