diff --git a/js.go b/js.go
index 34389d098..d14bfe278 100644
--- a/js.go
+++ b/js.go
@@ -260,6 +260,7 @@ type pubOpts struct {
 	lid string // Expected last msgId
 	str string // Expected stream name
 	seq uint64 // Expected last sequence
+	lss uint64 // Expected last sequence per subject
 }
 
 // pubAckResponse is the ack response from the JetStream API when publishing a message.
@@ -278,10 +279,11 @@ type PubAck struct {
 
 // Headers for published messages.
 const (
-	MsgIdHdr             = "Nats-Msg-Id"
-	ExpectedStreamHdr    = "Nats-Expected-Stream"
-	ExpectedLastSeqHdr   = "Nats-Expected-Last-Sequence"
-	ExpectedLastMsgIdHdr = "Nats-Expected-Last-Msg-Id"
+	MsgIdHdr               = "Nats-Msg-Id"
+	ExpectedStreamHdr      = "Nats-Expected-Stream"
+	ExpectedLastSeqHdr     = "Nats-Expected-Last-Sequence"
+	ExpectedLastSubjSeqHdr = "Nats-Expected-Last-Subject-Sequence"
+	ExpectedLastMsgIdHdr   = "Nats-Expected-Last-Msg-Id"
 )
 
 // PublishMsg publishes a Msg to a stream from JetStream.
@@ -317,6 +319,9 @@ func (js *js) PublishMsg(m *Msg, opts ...PubOpt) (*PubAck, error) {
 	if o.seq > 0 {
 		m.Header.Set(ExpectedLastSeqHdr, strconv.FormatUint(o.seq, 10))
 	}
+	if o.lss > 0 {
+		m.Header.Set(ExpectedLastSubjSeqHdr, strconv.FormatUint(o.lss, 10))
+	}
 
 	var resp *Msg
 	var err error
@@ -618,6 +623,9 @@ func (js *js) PublishMsgAsync(m *Msg, opts ...PubOpt) (PubAckFuture, error) {
 	if o.seq > 0 {
 		m.Header.Set(ExpectedLastSeqHdr, strconv.FormatUint(o.seq, 10))
 	}
+	if o.lss > 0 {
+		m.Header.Set(ExpectedLastSubjSeqHdr, strconv.FormatUint(o.lss, 10))
+	}
 
 	// Reply
 	if m.Reply != _EMPTY_ {
@@ -687,6 +695,14 @@ func ExpectLastSequence(seq uint64) PubOpt {
 	})
 }
 
+// ExpectLastSequencePerSubject sets the expected sequence per subject in the response from the publish.
+func ExpectLastSequencePerSubject(seq uint64) PubOpt {
+	return pubOptFn(func(opts *pubOpts) error {
+		opts.lss = seq
+		return nil
+	})
+}
+
 // ExpectLastMsgId sets the expected last msgId in the response from the publish.
 func ExpectLastMsgId(id string) PubOpt {
 	return pubOptFn(func(opts *pubOpts) error {
diff --git a/test/js_test.go b/test/js_test.go
index 660029d04..a54443042 100644
--- a/test/js_test.go
+++ b/test/js_test.go
@@ -264,6 +264,20 @@ func TestJetStreamPublish(t *testing.T) {
 	if err != context.Canceled {
 		t.Fatalf("Expected %q, got %q", context.Canceled, err)
 	}
+
+	// Test ExpectLastSequencePerSubject. Just make sure that we set the header.
+	sub, err = nc.SubscribeSync("test")
+	if err != nil {
+		t.Fatalf("Error on subscribe: %v", err)
+	}
+	js.Publish("test", []byte("msg"), nats.ExpectLastSequencePerSubject(1))
+	m, err := sub.NextMsg(time.Second)
+	if err != nil {
+		t.Fatalf("Error on next msg: %v", err)
+	}
+	if m.Header.Get(nats.ExpectedLastSubjSeqHdr) != "1" {
+		t.Fatalf("Header ExpectLastSequencePerSubject not set: %+v", m.Header)
+	}
 }
 
 func TestJetStreamSubscribe(t *testing.T) {