diff --git a/plugins/node/opentelemetry-instrumentation-mysql/src/instrumentation.ts b/plugins/node/opentelemetry-instrumentation-mysql/src/instrumentation.ts
index 82ea9770d4..8a0652d503 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql/src/instrumentation.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql/src/instrumentation.ts
@@ -14,7 +14,13 @@
  * limitations under the License.
  */
 
-import { diag, Span, SpanKind, SpanStatusCode } from '@opentelemetry/api';
+import {
+  context,
+  diag,
+  Span,
+  SpanKind,
+  SpanStatusCode,
+} from '@opentelemetry/api';
 import {
   InstrumentationBase,
   InstrumentationNodeModuleDefinition,
@@ -28,6 +34,11 @@ import { VERSION } from './version';
 
 type formatType = typeof mysqlTypes.format;
 
+type getConnectionCallbackType = (
+  err: mysqlTypes.MysqlError,
+  connection: mysqlTypes.PoolConnection
+) => void;
+
 export class MySQLInstrumentation extends InstrumentationBase<
   typeof mysqlTypes
 > {
@@ -176,21 +187,21 @@ export class MySQLInstrumentation extends InstrumentationBase<
 
         if (arguments.length === 1 && typeof arg1 === 'function') {
           const patchFn = thisPlugin._getConnectionCallbackPatchFn(
-            arg1,
+            arg1 as getConnectionCallbackType,
             format
           );
           return originalGetConnection.call(pool, patchFn);
         }
         if (arguments.length === 2 && typeof arg2 === 'function') {
           const patchFn = thisPlugin._getConnectionCallbackPatchFn(
-            arg2,
+            arg2 as getConnectionCallbackType,
             format
           );
           return originalGetConnection.call(pool, arg1, patchFn);
         }
         if (arguments.length === 3 && typeof arg3 === 'function') {
           const patchFn = thisPlugin._getConnectionCallbackPatchFn(
-            arg3,
+            arg3 as getConnectionCallbackType,
             format
           );
           return originalGetConnection.call(pool, arg1, arg2, patchFn);
@@ -201,22 +212,30 @@ export class MySQLInstrumentation extends InstrumentationBase<
     };
   }
 
-  private _getConnectionCallbackPatchFn(cb: Function, format: formatType) {
+  private _getConnectionCallbackPatchFn(
+    cb: getConnectionCallbackType,
+    format: formatType
+  ) {
     const thisPlugin = this;
-    return function () {
-      if (arguments[1]) {
+    const activeContext = context.active();
+    return function (
+      this: any,
+      err: mysqlTypes.MysqlError,
+      connection: mysqlTypes.PoolConnection
+    ) {
+      if (connection) {
         // this is the callback passed into a query
         // no need to unwrap
-        if (!isWrapped(arguments[1].query)) {
+        if (!isWrapped(connection.query)) {
           thisPlugin._wrap(
-            arguments[1],
+            connection,
             'query',
-            thisPlugin._patchQuery(arguments[1], format)
+            thisPlugin._patchQuery(connection, format)
           );
         }
       }
       if (typeof cb === 'function') {
-        cb(...arguments);
+        context.with(activeContext, cb, this, err, connection);
       }
     };
   }
diff --git a/plugins/node/opentelemetry-instrumentation-mysql/test/index.test.ts b/plugins/node/opentelemetry-instrumentation-mysql/test/index.test.ts
index fbf726281b..060b56e0cf 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql/test/index.test.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql/test/index.test.ts
@@ -426,6 +426,25 @@ describe('mysql@2.x', () => {
         });
       });
     });
+
+    it('should propagate active context to callback', done => {
+      const parentSpan = provider.getTracer('default').startSpan('test span');
+      context.with(trace.setSpan(context.active(), parentSpan), () => {
+        pool.getConnection((err, connection) => {
+          assert.ifError(err);
+          assert.ok(connection);
+          const sql = 'SELECT ? as solution';
+          connection.query(sql, 1, (err, res) => {
+            assert.ifError(err);
+            assert.ok(res);
+            assert.strictEqual(res[0].solution, 1);
+            const actualSpan = trace.getSpan(context.active());
+            assert.strictEqual(actualSpan, parentSpan);
+            done();
+          });
+        });
+      });
+    });
   });
 
   describe('#PoolCluster', () => {
@@ -600,6 +619,25 @@ describe('mysql@2.x', () => {
         }
       );
     });
+
+    it('should propagate active context to callback', done => {
+      const parentSpan = provider.getTracer('default').startSpan('test span');
+      context.with(trace.setSpan(context.active(), parentSpan), () => {
+        poolCluster.getConnection((err, connection) => {
+          assert.ifError(err);
+          assert.ok(connection);
+          const sql = 'SELECT ? as solution';
+          connection.query(sql, 1, (err, res) => {
+            assert.ifError(err);
+            assert.ok(res);
+            assert.strictEqual(res[0].solution, 1);
+            const actualSpan = trace.getSpan(context.active());
+            assert.strictEqual(actualSpan, parentSpan);
+            done();
+          });
+        });
+      });
+    });
   });
 });