diff --git a/plugins/web/opentelemetry-instrumentation-user-interaction/src/instrumentation.ts b/plugins/web/opentelemetry-instrumentation-user-interaction/src/instrumentation.ts
index 171527418b..da499ec1a9 100644
--- a/plugins/web/opentelemetry-instrumentation-user-interaction/src/instrumentation.ts
+++ b/plugins/web/opentelemetry-instrumentation-user-interaction/src/instrumentation.ts
@@ -267,7 +267,7 @@ export class UserInteractionInstrumentation extends InstrumentationBase<unknown>
         useCapture: any
       ) {
         const once = useCapture && useCapture.once;
-        const patchedListener = (...args: any[]) => {
+        const patchedListener = function (this: HTMLElement, ...args: any[]) {
           let parentSpan: api.Span | undefined;
           const event: Event | undefined = args[0];
           const target = event?.target;
@@ -285,14 +285,14 @@ export class UserInteractionInstrumentation extends InstrumentationBase<unknown>
             return api.context.with(
               api.trace.setSpan(api.context.active(), span),
               () => {
-                const result = plugin._invokeListener(listener, target, args);
+                const result = plugin._invokeListener(listener, this, args);
                 // no zone so end span immediately
                 span.end();
                 return result;
               }
             );
           } else {
-            return plugin._invokeListener(listener, target, args);
+            return plugin._invokeListener(listener, this, args);
           }
         };
         if (plugin.addPatchedListener(this, type, listener, patchedListener)) {
diff --git a/plugins/web/opentelemetry-instrumentation-user-interaction/test/userInteraction.nozone.test.ts b/plugins/web/opentelemetry-instrumentation-user-interaction/test/userInteraction.nozone.test.ts
index 7bdae3e4fc..08447543be 100644
--- a/plugins/web/opentelemetry-instrumentation-user-interaction/test/userInteraction.nozone.test.ts
+++ b/plugins/web/opentelemetry-instrumentation-user-interaction/test/userInteraction.nozone.test.ts
@@ -486,7 +486,11 @@ describe('UserInteractionInstrumentation', () => {
       btn2.setAttribute('id', 'btn2');
       root.appendChild(btn2);
 
-      root.addEventListener('click', event => {
+      const listenerThis: EventTarget[] = [];
+      root.addEventListener('click', function (event) {
+        // Assert here with failure would also affect other tests due to setTimeout bellow
+        listenerThis.push(this);
+
         switch (event.target) {
           case btn1:
             getData(FILE_URL, () => {
@@ -506,6 +510,17 @@ describe('UserInteractionInstrumentation', () => {
 
       sandbox.clock.tick(1000);
       originalSetTimeout(() => {
+        assert.strictEqual(
+          listenerThis[0],
+          root,
+          'this inside event listener matches listened target (0)'
+        );
+        assert.strictEqual(
+          listenerThis[1],
+          root,
+          'this inside event listener matches listened target (1)'
+        );
+
         assert.equal(exportSpy.args.length, 4, 'should export 4 spans');
 
         const span1: tracing.ReadableSpan = exportSpy.args[0][0][0];