diff --git a/.changeset/blue-carrots-think.md b/.changeset/blue-carrots-think.md
new file mode 100644
index 00000000000..964bf60b770
--- /dev/null
+++ b/.changeset/blue-carrots-think.md
@@ -0,0 +1,6 @@
+---
+"@pnpm/plugin-commands-patching": patch
+"pnpm": patch
+---
+
+`pnpm patch` should work on files that don't have an end of line [#5320](https://github.com/pnpm/pnpm/issues/5320).
diff --git a/packages/plugin-commands-patching/src/patchCommit.ts b/packages/plugin-commands-patching/src/patchCommit.ts
index 4c22b4102f0..cc4666d9991 100644
--- a/packages/plugin-commands-patching/src/patchCommit.ts
+++ b/packages/plugin-commands-patching/src/patchCommit.ts
@@ -98,4 +98,5 @@ async function diffFolders (folderA: string, folderB: string) {
     .replace(new RegExp(`(a|b)${escapeStringRegexp(`/${normalizePath(folderBN)}/`)}`, 'g'), '$1/')
     .replace(new RegExp(escapeStringRegexp(`${folderAN}/`), 'g'), '')
     .replace(new RegExp(escapeStringRegexp(`${folderBN}/`), 'g'), '')
+    .replace(/\n\\ No newline at end of file$/, '')
 }
diff --git a/packages/plugin-commands-patching/test/patch.test.ts b/packages/plugin-commands-patching/test/patch.test.ts
index c6b6169166a..949081c30bf 100644
--- a/packages/plugin-commands-patching/test/patch.test.ts
+++ b/packages/plugin-commands-patching/test/patch.test.ts
@@ -94,3 +94,80 @@ describe('patch and commit', () => {
       .rejects.toThrow(`The target directory already exists: '${editDir}'`)
   })
 })
+
+describe('patching should work when there is a no EOL in the patched file', () => {
+  let defaultPatchOption: patch.PatchCommandOptions
+
+  beforeEach(() => {
+    prepare({
+      dependencies: {
+        'safe-execa': '0.1.2',
+      },
+    })
+
+    const cacheDir = path.resolve('cache')
+    const storeDir = path.resolve('store')
+
+    defaultPatchOption = {
+      cacheDir,
+      dir: process.cwd(),
+      pnpmHomeDir: '',
+      rawConfig: {
+        registry: `http://localhost:${REGISTRY_MOCK_PORT}/`,
+      },
+      registries: { default: `http://localhost:${REGISTRY_MOCK_PORT}/` },
+      storeDir,
+      userConfig: {},
+    }
+  })
+  it('should work when adding content on a newline', async () => {
+    const output = await patch.handler(defaultPatchOption, ['safe-execa@0.1.2'])
+    const userPatchDir = output.substring(output.indexOf(':') + 1).trim()
+    const tempDir = os.tmpdir()
+
+    expect(userPatchDir).toContain(tempDir)
+    expect(fs.existsSync(userPatchDir)).toBe(true)
+    expect(fs.existsSync(path.join(userPatchDir, 'lib/index.js'))).toBe(true)
+
+    fs.appendFileSync(path.join(userPatchDir, 'lib/index.js'), '\n// test patching', 'utf8')
+
+    await patchCommit.handler({
+      ...DEFAULT_OPTS,
+      dir: process.cwd(),
+    }, [userPatchDir])
+
+    const { manifest } = await readProjectManifest(process.cwd())
+    expect(manifest.pnpm?.patchedDependencies).toStrictEqual({
+      'safe-execa@0.1.2': 'patches/safe-execa@0.1.2.patch',
+    })
+    const patchContent = fs.readFileSync('patches/safe-execa@0.1.2.patch', 'utf8')
+    expect(patchContent).toContain('diff --git')
+    expect(patchContent).toContain('// test patching')
+    expect(patchContent).not.toContain('No newline at end of file')
+    expect(fs.readFileSync('node_modules/safe-execa/lib/index.js', 'utf8')).toContain('// test patching')
+  })
+  it('should work fine when new content is appended', async () => {
+    const output = await patch.handler(defaultPatchOption, ['safe-execa@0.1.2'])
+    const userPatchDir = output.substring(output.indexOf(':') + 1).trim()
+    const tempDir = os.tmpdir()
+
+    expect(userPatchDir).toContain(tempDir)
+    expect(fs.existsSync(userPatchDir)).toBe(true)
+    expect(fs.existsSync(path.join(userPatchDir, 'lib/index.js'))).toBe(true)
+
+    fs.appendFileSync(path.join(userPatchDir, 'lib/index.js'), '// patch without newline', 'utf8')
+
+    await patchCommit.handler({
+      ...DEFAULT_OPTS,
+      dir: process.cwd(),
+    }, [userPatchDir])
+
+    const { manifest } = await readProjectManifest(process.cwd())
+    expect(manifest.pnpm?.patchedDependencies).toStrictEqual({
+      'safe-execa@0.1.2': 'patches/safe-execa@0.1.2.patch',
+    })
+    const patchContent = fs.readFileSync('patches/safe-execa@0.1.2.patch', 'utf8')
+    expect(patchContent).toContain('No newline at end of file')
+    expect(fs.readFileSync('node_modules/safe-execa/lib/index.js', 'utf8')).toContain('//# sourceMappingURL=index.js.map// patch without newline')
+  })
+})