diff --git a/kokoro/linux/dockerfile/test/ruby/Dockerfile b/kokoro/linux/dockerfile/test/ruby/Dockerfile
index 41bfedeb330f..9037da715f09 100644
--- a/kokoro/linux/dockerfile/test/ruby/Dockerfile
+++ b/kokoro/linux/dockerfile/test/ruby/Dockerfile
@@ -32,6 +32,7 @@ RUN /bin/bash -l -c "rvm install 2.3.8"
 RUN /bin/bash -l -c "rvm install 2.4.5"
 RUN /bin/bash -l -c "rvm install 2.5.1"
 RUN /bin/bash -l -c "rvm install 2.6.0"
+RUN /bin/bash -l -c "rvm install 2.7.0"
 
 RUN /bin/bash -l -c "echo 'gem: --no-ri --no-rdoc' > ~/.gemrc"
 RUN /bin/bash -l -c "echo 'export PATH=/usr/local/rvm/bin:$PATH' >> ~/.bashrc"
diff --git a/kokoro/linux/ruby27/build.sh b/kokoro/linux/ruby27/build.sh
new file mode 100755
index 000000000000..c38ee36e584a
--- /dev/null
+++ b/kokoro/linux/ruby27/build.sh
@@ -0,0 +1,18 @@
+#!/bin/bash
+#
+# This is the top-level script we give to Kokoro as the entry point for
+# running the "pull request" project:
+#
+# This script selects a specific Dockerfile (for building a Docker image) and
+# a script to run inside that image.  Then we delegate to the general
+# build_and_run_docker.sh script.
+
+# Change to repo root
+cd $(dirname $0)/../../..
+
+export DOCKERHUB_ORGANIZATION=protobuftesting
+export DOCKERFILE_DIR=kokoro/linux/dockerfile/test/ruby
+export DOCKER_RUN_SCRIPT=kokoro/linux/pull_request_in_docker.sh
+export OUTPUT_DIR=testoutput
+export TEST_SET="ruby27"
+./kokoro/linux/build_and_run_docker.sh
diff --git a/kokoro/linux/ruby27/continuous.cfg b/kokoro/linux/ruby27/continuous.cfg
new file mode 100644
index 000000000000..9cce8c90e1ff
--- /dev/null
+++ b/kokoro/linux/ruby27/continuous.cfg
@@ -0,0 +1,11 @@
+# Config file for running tests in Kokoro
+
+# Location of the build script in repository
+build_file: "protobuf/kokoro/linux/ruby27/build.sh"
+timeout_mins: 120
+
+action {
+  define_artifacts {
+    regex: "**/sponge_log.xml"
+  }
+}
diff --git a/kokoro/linux/ruby27/presubmit.cfg b/kokoro/linux/ruby27/presubmit.cfg
new file mode 100644
index 000000000000..9cce8c90e1ff
--- /dev/null
+++ b/kokoro/linux/ruby27/presubmit.cfg
@@ -0,0 +1,11 @@
+# Config file for running tests in Kokoro
+
+# Location of the build script in repository
+build_file: "protobuf/kokoro/linux/ruby27/build.sh"
+timeout_mins: 120
+
+action {
+  define_artifacts {
+    regex: "**/sponge_log.xml"
+  }
+}
diff --git a/kokoro/macos/ruby27/build.sh b/kokoro/macos/ruby27/build.sh
new file mode 100755
index 000000000000..16bcbd6cbd6f
--- /dev/null
+++ b/kokoro/macos/ruby27/build.sh
@@ -0,0 +1,11 @@
+#!/bin/bash
+#
+# Build file to set up and run tests
+
+# Change to repo root
+cd $(dirname $0)/../../..
+
+# Prepare worker environment to run tests
+source kokoro/macos/prepare_build_macos_rc
+
+./tests.sh ruby27
diff --git a/kokoro/macos/ruby27/continuous.cfg b/kokoro/macos/ruby27/continuous.cfg
new file mode 100644
index 000000000000..b10b455da3fb
--- /dev/null
+++ b/kokoro/macos/ruby27/continuous.cfg
@@ -0,0 +1,5 @@
+# Config file for running tests in Kokoro
+
+# Location of the build script in repository
+build_file: "protobuf/kokoro/macos/ruby27/build.sh"
+timeout_mins: 1440
diff --git a/kokoro/macos/ruby27/presubmit.cfg b/kokoro/macos/ruby27/presubmit.cfg
new file mode 100644
index 000000000000..b10b455da3fb
--- /dev/null
+++ b/kokoro/macos/ruby27/presubmit.cfg
@@ -0,0 +1,5 @@
+# Config file for running tests in Kokoro
+
+# Location of the build script in repository
+build_file: "protobuf/kokoro/macos/ruby27/build.sh"
+timeout_mins: 1440
diff --git a/ruby/compatibility_tests/v3.0.0/tests/repeated_field_test.rb b/ruby/compatibility_tests/v3.0.0/tests/repeated_field_test.rb
index 4ebb731a83f6..b4a158f37ccd 100755
--- a/ruby/compatibility_tests/v3.0.0/tests/repeated_field_test.rb
+++ b/ruby/compatibility_tests/v3.0.0/tests/repeated_field_test.rb
@@ -20,6 +20,7 @@ def test_acts_like_an_array
       :iter_for_each_with_index, :dimensions, :copy_data, :copy_data_simple,
       :nitems, :iter_for_reverse_each, :indexes, :append, :prepend]
     arr_methods -= [:union, :difference, :filter!]
+    arr_methods -= [:intersection, :deconstruct] # ruby 2.7 methods we can ignore
     arr_methods.each do |method_name|
       assert m.repeated_string.respond_to?(method_name) == true, "does not respond to #{method_name}"
     end
diff --git a/ruby/tests/gc_test.rb b/ruby/tests/gc_test.rb
index 55b96289e812..6ef4e2e301ed 100755
--- a/ruby/tests/gc_test.rb
+++ b/ruby/tests/gc_test.rb
@@ -4,7 +4,9 @@
 $LOAD_PATH.unshift(File.expand_path(File.dirname(__FILE__)))
 
 old_gc = GC.stress
-GC.stress = 0x01 | 0x04
+# Ruby 2.7.0 - 2.7.1 has a GC bug in its parser, so turn off stress for now
+# See https://bugs.ruby-lang.org/issues/16807
+GC.stress = 0x01 | 0x04 unless RUBY_VERSION.match?(/^2\.7\./)
 require 'generated_code_pb'
 require 'generated_code_proto2_pb'
 GC.stress = old_gc
diff --git a/ruby/travis-test.sh b/ruby/travis-test.sh
index 6dec0c906aa5..b39a6c5d121b 100755
--- a/ruby/travis-test.sh
+++ b/ruby/travis-test.sh
@@ -16,7 +16,7 @@ test_version() {
        git clean -f && \
        gem install bundler && bundle && \
        rake test"
-  elif [ "$version" == "ruby-2.6.0" ] ; then
+  elif [ "$version" == "ruby-2.6.0" -o "$version" == "ruby-2.7.0" ] ; then
     bash --login -c \
       "rvm install $version && rvm use $version && \
        which ruby && \
diff --git a/tests.sh b/tests.sh
index 84fb0b124b21..faaec76f0aa8 100755
--- a/tests.sh
+++ b/tests.sh
@@ -919,6 +919,7 @@ Usage: $0 { cpp |
             ruby24 |
             ruby25 |
             ruby26 |
+            ruby27 |
             jruby |
             ruby_all |
             php5.5   |