diff --git a/changelog/9163.bugfix.rst b/changelog/9163.bugfix.rst
new file mode 100644
index 00000000000..fb559d10fe8
--- /dev/null
+++ b/changelog/9163.bugfix.rst
@@ -0,0 +1 @@
+The end line number and end column offset are now properly set for rewritten assert statements.
diff --git a/src/_pytest/assertion/rewrite.py b/src/_pytest/assertion/rewrite.py
index 99416aed20b..1f7de90d5a9 100644
--- a/src/_pytest/assertion/rewrite.py
+++ b/src/_pytest/assertion/rewrite.py
@@ -19,6 +19,7 @@
 from typing import Dict
 from typing import IO
 from typing import Iterable
+from typing import Iterator
 from typing import List
 from typing import Optional
 from typing import Sequence
@@ -539,19 +540,11 @@ def _check_if_assertion_pass_impl() -> bool:
 }
 
 
-def set_location(node, lineno, col_offset):
-    """Set node location information recursively."""
-
-    def _fix(node, lineno, col_offset):
-        if "lineno" in node._attributes:
-            node.lineno = lineno
-        if "col_offset" in node._attributes:
-            node.col_offset = col_offset
-        for child in ast.iter_child_nodes(node):
-            _fix(child, lineno, col_offset)
-
-    _fix(node, lineno, col_offset)
-    return node
+def traverse_node(node: ast.AST) -> Iterator[ast.AST]:
+    """Recursively yield node and all its children in depth-first order."""
+    yield node
+    for child in ast.iter_child_nodes(node):
+        yield from traverse_node(child)
 
 
 @functools.lru_cache(maxsize=1)
@@ -954,9 +947,10 @@ def visit_Assert(self, assert_: ast.Assert) -> List[ast.stmt]:
             variables = [ast.Name(name, ast.Store()) for name in self.variables]
             clear = ast.Assign(variables, ast.NameConstant(None))
             self.statements.append(clear)
-        # Fix line numbers.
+        # Fix locations (line numbers/column offsets).
         for stmt in self.statements:
-            set_location(stmt, assert_.lineno, assert_.col_offset)
+            for node in traverse_node(stmt):
+                ast.copy_location(node, assert_)
         return self.statements
 
     def visit_Name(self, name: ast.Name) -> Tuple[ast.Name, str]:
diff --git a/testing/test_assertrewrite.py b/testing/test_assertrewrite.py
index d732cbdd709..5e63d61fa30 100644
--- a/testing/test_assertrewrite.py
+++ b/testing/test_assertrewrite.py
@@ -111,6 +111,28 @@ def test_place_initial_imports(self) -> None:
             assert imp.col_offset == 0
         assert isinstance(m.body[3], ast.Expr)
 
+    def test_location_is_set(self) -> None:
+        s = textwrap.dedent(
+            """
+
+        assert False, (
+
+            "Ouch"
+          )
+
+        """
+        )
+        m = rewrite(s)
+        for node in m.body:
+            if isinstance(node, ast.Import):
+                continue
+            for n in [node, *ast.iter_child_nodes(node)]:
+                assert n.lineno == 3
+                assert n.col_offset == 0
+                if sys.version_info >= (3, 8):
+                    assert n.end_lineno == 6
+                    assert n.end_col_offset == 3
+
     def test_dont_rewrite(self) -> None:
         s = """'PYTEST_DONT_REWRITE'\nassert 14"""
         m = rewrite(s)