diff --git a/Tests/test_file_apng.py b/Tests/test_file_apng.py
index 8cb9a814ea5..57928880882 100644
--- a/Tests/test_file_apng.py
+++ b/Tests/test_file_apng.py
@@ -673,10 +673,16 @@ def test_seek_after_close():
 
 
 @pytest.mark.parametrize("mode", ("RGBA", "RGB", "P"))
-def test_different_modes_in_later_frames(mode, tmp_path):
+@pytest.mark.parametrize("default_image", (True, False))
+def test_different_modes_in_later_frames(mode, default_image, tmp_path):
     test_file = str(tmp_path / "temp.png")
 
     im = Image.new("L", (1, 1))
-    im.save(test_file, save_all=True, append_images=[Image.new(mode, (1, 1))])
+    im.save(
+        test_file,
+        save_all=True,
+        default_image=default_image,
+        append_images=[Image.new(mode, (1, 1))],
+    )
     with Image.open(test_file) as reloaded:
         assert reloaded.mode == mode
diff --git a/src/PIL/PngImagePlugin.py b/src/PIL/PngImagePlugin.py
index 2c7ae68d53d..5e5a8cf6a2d 100644
--- a/src/PIL/PngImagePlugin.py
+++ b/src/PIL/PngImagePlugin.py
@@ -1105,10 +1105,7 @@ def _write_multiple_frames(im, fp, chunk, rawmode, default_image, append_images)
             if im_frame.mode == rawmode:
                 im_frame = im_frame.copy()
             else:
-                if rawmode == "P":
-                    im_frame = im_frame.convert(rawmode, palette=im.palette)
-                else:
-                    im_frame = im_frame.convert(rawmode)
+                im_frame = im_frame.convert(rawmode)
             encoderinfo = im.encoderinfo.copy()
             if isinstance(duration, (list, tuple)):
                 encoderinfo["duration"] = duration[frame_count]
@@ -1167,6 +1164,8 @@ def _write_multiple_frames(im, fp, chunk, rawmode, default_image, append_images)
 
     # default image IDAT (if it exists)
     if default_image:
+        if im.mode != rawmode:
+            im = im.convert(rawmode)
         ImageFile._save(im, _idat(fp, chunk), [("zip", (0, 0) + im.size, 0, rawmode)])
 
     seq_num = 0
@@ -1228,11 +1227,7 @@ def _save(im, fp, filename, chunk=putchunk, save_all=False):
         )
         modes = set()
         append_images = im.encoderinfo.get("append_images", [])
-        if default_image:
-            chain = itertools.chain(append_images)
-        else:
-            chain = itertools.chain([im], append_images)
-        for im_seq in chain:
+        for im_seq in itertools.chain([im], append_images):
             for im_frame in ImageSequence.Iterator(im_seq):
                 modes.add(im_frame.mode)
         for mode in ("RGBA", "RGB", "P"):