diff --git a/lib/plugins/aws/invokeLocal/index.js b/lib/plugins/aws/invokeLocal/index.js
index d0e2d473893..113990d1312 100644
--- a/lib/plugins/aws/invokeLocal/index.js
+++ b/lib/plugins/aws/invokeLocal/index.js
@@ -370,7 +370,9 @@ class AwsInvokeLocal {
 
     let dockerFileContent = `FROM lambci/lambda:${runtime}`;
     for (const layerPath of layerPaths) {
-      dockerFileContent += `\nADD --chown=sbx_user1051:495 ${layerPath} /opt`;
+      dockerFileContent += `\nADD --chown=sbx_user1051:495 ${
+        os.platform() === 'win32' ? layerPath.replace(/\\/g, '/') : layerPath
+      } /opt`;
     }
 
     const dockerfilePath = path.join('.serverless', 'invokeLocal', runtime, 'Dockerfile');