diff --git a/packages/jest-runner/src/jest-test-adapters/jest-greater-than-25-adapter.ts b/packages/jest-runner/src/jest-test-adapters/jest-greater-than-25-adapter.ts
index 9a5fc1503b..b264f347da 100644
--- a/packages/jest-runner/src/jest-test-adapters/jest-greater-than-25-adapter.ts
+++ b/packages/jest-runner/src/jest-test-adapters/jest-greater-than-25-adapter.ts
@@ -1,11 +1,14 @@
+import { join } from 'path';
+
 import { jestWrapper } from '../utils';
 import { JestRunResult } from '../jest-run-result';
 
 import { JestTestAdapter, RunSettings } from './jest-test-adapter';
 
 export class JestGreaterThan25TestAdapter implements JestTestAdapter {
-  public async run({ jestConfig, projectRoot, fileNameUnderTest, testNamePattern }: RunSettings): Promise<JestRunResult> {
-    const config = JSON.stringify(jestConfig);
+  public async run({ jestConfig, projectRoot, fileNameUnderTest, testNamePattern, jestConfigPath }: RunSettings): Promise<JestRunResult> {
+    const config = jestConfigPath ? join(projectRoot, jestConfigPath) : JSON.stringify(jestConfig);
+
     const result = await jestWrapper.runCLI(
       {
         $0: 'stryker',
diff --git a/packages/jest-runner/src/jest-test-adapters/jest-less-than-25-adapter.ts b/packages/jest-runner/src/jest-test-adapters/jest-less-than-25-adapter.ts
index 72a4820022..b93fb1bd46 100644
--- a/packages/jest-runner/src/jest-test-adapters/jest-less-than-25-adapter.ts
+++ b/packages/jest-runner/src/jest-test-adapters/jest-less-than-25-adapter.ts
@@ -1,3 +1,4 @@
+import { join } from 'path';
 import jest from 'jest';
 
 import { JestRunResult } from '../jest-run-result';
@@ -9,8 +10,8 @@ import { RunSettings, JestTestAdapter } from './jest-test-adapter';
  * It has a lot of `any` typings here, since the installed typings are not in sync.
  */
 export class JestLessThan25TestAdapter implements JestTestAdapter {
-  public run({ jestConfig, projectRoot, fileNameUnderTest, testNamePattern }: RunSettings): Promise<JestRunResult> {
-    const config = JSON.stringify(jestConfig);
+  public run({ jestConfig, projectRoot, fileNameUnderTest, testNamePattern, jestConfigPath }: RunSettings): Promise<JestRunResult> {
+    const config = jestConfigPath ? join(projectRoot, jestConfigPath) : JSON.stringify(jestConfig);
     return jest.runCLI(
       {
         ...(fileNameUnderTest && { _: [fileNameUnderTest], findRelatedTests: true }),
diff --git a/packages/jest-runner/src/jest-test-adapters/jest-test-adapter.ts b/packages/jest-runner/src/jest-test-adapters/jest-test-adapter.ts
index 73970ee65f..cf3a6034ae 100644
--- a/packages/jest-runner/src/jest-test-adapters/jest-test-adapter.ts
+++ b/packages/jest-runner/src/jest-test-adapters/jest-test-adapter.ts
@@ -5,6 +5,7 @@ import { JestRunResult } from '../jest-run-result';
 export interface RunSettings {
   jestConfig: Config.InitialOptions;
   projectRoot: string;
+  jestConfigPath?: string;
   testNamePattern?: string;
   fileNameUnderTest?: string;
 }
diff --git a/packages/jest-runner/src/jest-test-runner.ts b/packages/jest-runner/src/jest-test-runner.ts
index a713063cd4..7e174dbd44 100644
--- a/packages/jest-runner/src/jest-test-runner.ts
+++ b/packages/jest-runner/src/jest-test-runner.ts
@@ -60,6 +60,7 @@ export const jestTestRunnerFactory = createJestTestRunnerFactory();
 export class JestTestRunner implements TestRunner {
   private readonly jestConfig: jest.Config.InitialOptions;
   private readonly enableFindRelatedTests: boolean;
+  private readonly jestRunnerOptions: JestRunnerOptionsWithStrykerOptions;
 
   public static inject = tokens(
     commonTokens.logger,
@@ -78,13 +79,13 @@ export class JestTestRunner implements TestRunner {
     configLoader: JestConfigLoader,
     private readonly globalNamespace: typeof INSTRUMENTER_CONSTANTS.NAMESPACE | '__stryker2__'
   ) {
-    const jestOptions = options as JestRunnerOptionsWithStrykerOptions;
+    this.jestRunnerOptions = options as JestRunnerOptionsWithStrykerOptions;
     // Get jest configuration from stryker options and assign it to jestConfig
     const configFromFile = configLoader.loadConfig();
-    this.jestConfig = this.mergeConfigSettings(configFromFile, jestOptions.jest || {});
+    this.jestConfig = this.mergeConfigSettings(configFromFile, this.jestRunnerOptions.jest || {});
 
     // Get enableFindRelatedTests from stryker jest options or default to true
-    this.enableFindRelatedTests = jestOptions.jest.enableFindRelatedTests;
+    this.enableFindRelatedTests = this.jestRunnerOptions.jest.enableFindRelatedTests;
 
     if (this.enableFindRelatedTests) {
       this.log.debug('Running jest with --findRelatedTests flag. Set jest.enableFindRelatedTests to false to run all tests on every mutant.');
@@ -109,6 +110,7 @@ export class JestTestRunner implements TestRunner {
       const { dryRunResult, jestResult } = await this.run({
         jestConfig: withCoverageAnalysis(this.jestConfig, coverageAnalysis),
         projectRoot: process.cwd(),
+        jestConfigPath: this.jestRunnerOptions.jest?.configFile,
       });
       if (dryRunResult.status === DryRunStatus.Complete && coverageAnalysis !== 'off') {
         const errorMessage = verifyAllTestFilesHaveCoverage(jestResult, fileNamesWithMutantCoverage);
@@ -132,11 +134,17 @@ export class JestTestRunner implements TestRunner {
     state.coverageAnalysis = 'off';
     let testNamePattern: string | undefined;
     if (testFilter) {
-      testNamePattern = testFilter.map((testId) => `(${escapeRegExp(testId)})`).join('|');
+      testNamePattern = testFilter.map(testId => `(${escapeRegExp(testId)})`).join('|');
     }
     process.env[INSTRUMENTER_CONSTANTS.ACTIVE_MUTANT_ENV_VARIABLE] = activeMutant.id.toString();
     try {
-      const { dryRunResult } = await this.run({ fileNameUnderTest, jestConfig: this.jestConfig, projectRoot: process.cwd(), testNamePattern });
+      const { dryRunResult } = await this.run({
+        fileNameUnderTest,
+        jestConfig: this.jestConfig,
+        projectRoot: process.cwd(),
+        testNamePattern,
+        jestConfigPath: this.jestRunnerOptions.jest?.configFile,
+      });
       return toMutantRunResult(dryRunResult);
     } finally {
       delete process.env[INSTRUMENTER_CONSTANTS.ACTIVE_MUTANT_ENV_VARIABLE];
@@ -155,7 +163,7 @@ export class JestTestRunner implements TestRunner {
   private collectRunResult(results: jestTestResult.AggregatedResult): DryRunResult {
     if (results.numRuntimeErrorTestSuites) {
       const errorMessage = results.testResults
-        .map((testSuite) => this.collectSerializableErrorText(testSuite.testExecError))
+        .map(testSuite => this.collectSerializableErrorText(testSuite.testExecError))
         .filter(notEmpty)
         .join(', ');
       return {
diff --git a/packages/jest-runner/test/unit/jest-test-adapters/jest-greater-than-25-adapter.spec.ts b/packages/jest-runner/test/unit/jest-test-adapters/jest-greater-than-25-adapter.spec.ts
index 3f1a0e5828..7acc294d88 100644
--- a/packages/jest-runner/test/unit/jest-test-adapters/jest-greater-than-25-adapter.spec.ts
+++ b/packages/jest-runner/test/unit/jest-test-adapters/jest-greater-than-25-adapter.spec.ts
@@ -13,6 +13,7 @@ describe(JestGreaterThan25TestAdapter.name, () => {
 
   const projectRoot = '/path/to/project';
   const fileNameUnderTest = '/path/to/file';
+  const jestConfigPath = 'jest.config.js';
   const jestConfig: Config.InitialOptions = { rootDir: projectRoot };
 
   beforeEach(() => {
@@ -30,6 +31,42 @@ describe(JestGreaterThan25TestAdapter.name, () => {
     expect(runCLIStub).calledWith(sinon.match.object, [projectRoot]);
   });
 
+  describe('when jestConfigPath not provided', () => {
+    it('should call the runCLI method with the stringified jest config flag', async () => {
+      await sut.run({ jestConfig, projectRoot, fileNameUnderTest });
+
+      expect(runCLIStub).calledWith(
+        {
+          $0: 'stryker',
+          _: [fileNameUnderTest],
+          config: JSON.stringify({ rootDir: projectRoot }),
+          findRelatedTests: true,
+          runInBand: true,
+          silent: true,
+          testNamePattern: undefined,
+        },
+        [projectRoot]
+      );
+    });
+  });
+  describe('when jestConfigPath provided', () => {
+    it('should pass the config path instead jest config flag', async () => {
+      await sut.run({ jestConfig, projectRoot, fileNameUnderTest, jestConfigPath });
+
+      expect(runCLIStub).calledWith(
+        {
+          $0: 'stryker',
+          _: [fileNameUnderTest],
+          config: `${projectRoot}/${jestConfigPath}`,
+          findRelatedTests: true,
+          runInBand: true,
+          silent: true,
+          testNamePattern: undefined,
+        },
+        [projectRoot]
+      );
+    });
+  });
   it('should call the runCLI method with the --findRelatedTests flag', async () => {
     await sut.run({ jestConfig, projectRoot, fileNameUnderTest });