diff --git a/packages/vite/src/node/server/transformRequest.ts b/packages/vite/src/node/server/transformRequest.ts
index 0bab9703c075c7..c7bf13ca0929ab 100644
--- a/packages/vite/src/node/server/transformRequest.ts
+++ b/packages/vite/src/node/server/transformRequest.ts
@@ -295,7 +295,7 @@ async function loadAndTransform(
           // to resolve and display them in a meaningful way (rather than
           // with absolute paths).
           if (path.isAbsolute(sourcePath)) {
-            map.sourceRoot = path.dirname(mod.file) + path.sep
+            map.sourceRoot = path.dirname(mod.file)
             map.sources[sourcesIndex] = path.relative(
               path.dirname(mod.file),
               sourcePath,
diff --git a/playground/css-sourcemap/__tests__/css-sourcemap.spec.ts b/playground/css-sourcemap/__tests__/css-sourcemap.spec.ts
index 5be3c0a23b3e24..98dad19c1b9683 100644
--- a/playground/css-sourcemap/__tests__/css-sourcemap.spec.ts
+++ b/playground/css-sourcemap/__tests__/css-sourcemap.spec.ts
@@ -69,7 +69,7 @@ describe.runIf(isServe)('serve', () => {
     expect(formatSourcemapForSnapshot(map)).toMatchInlineSnapshot(`
       {
         "mappings": "AAAA;EACE,UAAU;AACZ;;ACAA;EACE,UAAU;AACZ",
-        "sourceRoot": "/root/",
+        "sourceRoot": "/root",
         "sources": [
           "be-imported.css",
           "linked-with-import.css",
diff --git a/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts b/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts
index b86af9df81aeca..2908faca709fcd 100644
--- a/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts
+++ b/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts
@@ -24,7 +24,7 @@ if (!isBuild) {
     expect(formatSourcemapForSnapshot(map)).toMatchInlineSnapshot(`
       {
         "mappings": "AAAO,aAAM,MAAM;",
-        "sourceRoot": "/root/",
+        "sourceRoot": "/root",
         "sources": [
           "bar.ts",
         ],
diff --git a/playground/ssr-html/__tests__/ssr-html.spec.ts b/playground/ssr-html/__tests__/ssr-html.spec.ts
index 2bb082aa7407c2..e4360f698292bf 100644
--- a/playground/ssr-html/__tests__/ssr-html.spec.ts
+++ b/playground/ssr-html/__tests__/ssr-html.spec.ts
@@ -3,6 +3,7 @@ import { promisify } from 'node:util'
 import path from 'node:path'
 import fetch from 'node-fetch'
 import { describe, expect, test } from 'vitest'
+import { normalizePath } from 'vite'
 import { port } from './serve'
 import { editFile, isServe, page, untilUpdated } from '~utils'
 
@@ -83,10 +84,14 @@ describe.runIf(isServe)('stacktrace', () => {
 
         const reg = new RegExp(
           // TODO: ts without sourcemaps will resolve column to 8 which should be 9
-          path.resolve(__dirname, '../src') + '/error\\.' + ext + ':2:[89]',
+          normalizePath(
+            path.resolve(__dirname, '../src', `error.${ext}`),
+          ).replace('.', '\\.') + ':2:[89]',
+          'i',
         )
 
         lines.forEach((line) => {
+          console.log(line.trim())
           expect(line.trim()).toMatch(reg)
         })
       })
diff --git a/playground/test-utils.ts b/playground/test-utils.ts
index 9bfbb2d550851b..048f6c8dd9b0e7 100644
--- a/playground/test-utils.ts
+++ b/playground/test-utils.ts
@@ -308,7 +308,7 @@ export const formatSourcemapForSnapshot = (map: any): any => {
   delete m.names
   m.sources = m.sources.map((source) => source.replace(root, '/root'))
   if (m.sourceRoot) {
-    m.sourceRoot = m.sourceRoot.replace(root + path.sep, '/root/')
+    m.sourceRoot = m.sourceRoot.replace(root, '/root')
   }
   return m
 }