diff --git a/packages/vite/src/client/client.ts b/packages/vite/src/client/client.ts
index cc4cd0c5731291..5c04bc23af0cc1 100644
--- a/packages/vite/src/client/client.ts
+++ b/packages/vite/src/client/client.ts
@@ -12,7 +12,6 @@ import { ErrorOverlay, overlayId } from './overlay'
 import '@vite/env'
 
 // injected by the hmr plugin when served
-declare const __ROOT__: string
 declare const __BASE__: string
 declare const __HMR_PROTOCOL__: string
 declare const __HMR_HOSTNAME__: string
diff --git a/packages/vite/src/node/plugins/clientInjections.ts b/packages/vite/src/node/plugins/clientInjections.ts
index 17d48f1bb5c894..ce8cdb25795a66 100644
--- a/packages/vite/src/node/plugins/clientInjections.ts
+++ b/packages/vite/src/node/plugins/clientInjections.ts
@@ -43,7 +43,6 @@ export function clientInjectionsPlugin(config: ResolvedConfig): Plugin {
         return code
           .replace(`__MODE__`, JSON.stringify(config.mode))
           .replace(`__BASE__`, JSON.stringify(config.base))
-          .replace(`__ROOT__`, JSON.stringify(config.root))
           .replace(`__DEFINES__`, serializeDefine(config.define || {}))
           .replace(`__HMR_PROTOCOL__`, JSON.stringify(protocol))
           .replace(`__HMR_HOSTNAME__`, JSON.stringify(host))