diff --git a/packages/vite/src/node/plugins/resolve.ts b/packages/vite/src/node/plugins/resolve.ts
index 7a85aa84b24426..92965078e9e816 100644
--- a/packages/vite/src/node/plugins/resolve.ts
+++ b/packages/vite/src/node/plugins/resolve.ts
@@ -20,7 +20,6 @@ import {
   cleanUrl,
   createDebugger,
   deepImportRE,
-  ensureVolumeInPath,
   fsPathFromId,
   injectQuery,
   isBuiltin,
@@ -203,7 +202,7 @@ export function resolvePlugin(resolveOptions: InternalResolveOptions): Plugin {
       if (asSrc && depsOptimizer?.isOptimizedDepUrl(id)) {
         const optimizedPath = id.startsWith(FS_PREFIX)
           ? fsPathFromId(id)
-          : normalizePath(ensureVolumeInPath(path.resolve(root, id.slice(1))))
+          : normalizePath(path.resolve(root, id.slice(1)))
         return optimizedPath
       }
 
@@ -1250,7 +1249,6 @@ function equalWithoutSuffix(path: string, key: string, suffix: string) {
 }
 
 function getRealPath(resolved: string, preserveSymlinks?: boolean): string {
-  resolved = ensureVolumeInPath(resolved)
   if (!preserveSymlinks && browserExternalId !== resolved) {
     resolved = safeRealpathSync(resolved)
   }
diff --git a/packages/vite/src/node/server/middlewares/transform.ts b/packages/vite/src/node/server/middlewares/transform.ts
index 8fb184181c417d..b149c8e89fe9eb 100644
--- a/packages/vite/src/node/server/middlewares/transform.ts
+++ b/packages/vite/src/node/server/middlewares/transform.ts
@@ -7,7 +7,6 @@ import type { ViteDevServer } from '..'
 import {
   cleanUrl,
   createDebugger,
-  ensureVolumeInPath,
   fsPathFromId,
   injectQuery,
   isImportRequest,
@@ -79,9 +78,7 @@ export function transformMiddleware(
           // means that the dependency has already been pre-bundled and loaded
           const sourcemapPath = url.startsWith(FS_PREFIX)
             ? fsPathFromId(url)
-            : normalizePath(
-                ensureVolumeInPath(path.resolve(root, url.slice(1))),
-              )
+            : normalizePath(path.resolve(root, url.slice(1)))
           try {
             const map = JSON.parse(
               await fsp.readFile(sourcemapPath, 'utf-8'),
diff --git a/packages/vite/src/node/utils.ts b/packages/vite/src/node/utils.ts
index 68f26d95cf18cc..3f52cda0e53565 100644
--- a/packages/vite/src/node/utils.ts
+++ b/packages/vite/src/node/utils.ts
@@ -255,10 +255,6 @@ export function isParentDirectory(dir: string, file: string): boolean {
   )
 }
 
-export function ensureVolumeInPath(file: string): string {
-  return isWindows ? path.resolve(file) : file
-}
-
 export const queryRE = /\?.*$/s
 
 const postfixRE = /[?#].*$/s