diff --git a/packages/vite/src/node/__tests__/env.spec.ts b/packages/vite/src/node/__tests__/env.spec.ts
index 4e2a88f95b1665..e50083cf24f9a3 100644
--- a/packages/vite/src/node/__tests__/env.spec.ts
+++ b/packages/vite/src/node/__tests__/env.spec.ts
@@ -35,7 +35,6 @@ describe('loadEnv', () => {
         {
           "VITE_APP_BASE_ROUTE": "/app/",
           "VITE_APP_BASE_URL": "/app/",
-          "VITE_USER_NODE_ENV": "production",
         }
       `)
   })