diff --git a/packages/vite/src/node/plugins/importAnalysisBuild.ts b/packages/vite/src/node/plugins/importAnalysisBuild.ts
index 20bcb6449e12f2..ea79c21b13ca71 100644
--- a/packages/vite/src/node/plugins/importAnalysisBuild.ts
+++ b/packages/vite/src/node/plugins/importAnalysisBuild.ts
@@ -19,6 +19,7 @@ import type { ResolvedConfig } from '../config'
 import { toOutputFilePathInJS } from '../build'
 import { genSourceMapUrl } from '../server/sourcemap'
 import { getDepsOptimizer, optimizedDepNeedsInterop } from '../optimizer'
+import { SPECIAL_QUERY_RE } from '../constants'
 import { isCSSRequest, removedPureCssFilesCache } from './css'
 import { interopNamedImports } from './importAnalysis'
 
@@ -362,6 +363,8 @@ export function buildImportAnalysisPlugin(config: ResolvedConfig): Plugin {
           (source.slice(expStart, start).includes('from') || isDynamicImport) &&
           // already has ?used query (by import.meta.glob)
           !specifier.match(/\?used(&|$)/) &&
+          // don't append ?used when SPECIAL_QUERY_RE exists
+          !specifier.match(SPECIAL_QUERY_RE) &&
           // edge case for package names ending with .css (e.g normalize.css)
           !(bareImportRE.test(specifier) && !specifier.includes('/'))
         ) {
diff --git a/playground/assets/__tests__/relative-base/relative-base-assets.spec.ts b/playground/assets/__tests__/relative-base/relative-base-assets.spec.ts
index 70cb0fef940978..7f1df03bfcf42e 100644
--- a/playground/assets/__tests__/relative-base/relative-base-assets.spec.ts
+++ b/playground/assets/__tests__/relative-base/relative-base-assets.spec.ts
@@ -9,6 +9,8 @@ import {
   viteConfig,
 } from '~utils'
 
+const getBase = () => (viteConfig ? viteConfig?.testConfig?.baseRoute : '')
+
 const absoluteAssetMatch = isBuild
   ? /http.*\/other-assets\/asset-\w{8}\.png/
   : '/nested/asset.png'
@@ -138,7 +140,7 @@ describe('css url() references', () => {
 describe.runIf(isBuild)('index.css URLs', () => {
   let css: string
   beforeAll(() => {
-    const base = viteConfig ? viteConfig?.testConfig?.baseRoute : ''
+    const base = getBase()
     css = findAssetFile(/index.*\.css$/, base, 'other-assets')
   })
 
@@ -200,6 +202,10 @@ test('?url import on css', async () => {
   expect(txt).toMatch(
     isBuild ? /http.*\/other-assets\/icons-\w{8}\.css/ : '/css/icons.css',
   )
+  isBuild &&
+    expect(findAssetFile(/index.*\.js$/, getBase(), 'entries')).toMatch(
+      /icons-.+\.css(?!\?used)/,
+    )
 })
 
 test('new URL(..., import.meta.url)', async () => {