diff --git a/packages/vite/src/node/plugins/html.ts b/packages/vite/src/node/plugins/html.ts
index c33811008ccb17..bf3d662066630b 100644
--- a/packages/vite/src/node/plugins/html.ts
+++ b/packages/vite/src/node/plugins/html.ts
@@ -722,8 +722,6 @@ export function resolveHtmlTransforms(
   return [preHooks, postHooks]
 }
 
-export const maybeVirtualHtmlSet = new Set<string>()
-
 export async function applyHtmlTransforms(
   html: string,
   hooks: IndexHtmlTransformHook[],
@@ -734,8 +732,6 @@ export async function applyHtmlTransforms(
   const bodyTags: HtmlTagDescriptor[] = []
   const bodyPrependTags: HtmlTagDescriptor[] = []
 
-  maybeVirtualHtmlSet.add(ctx.filename)
-
   for (const hook of hooks) {
     const res = await hook(html, ctx)
     if (!res) {
diff --git a/packages/vite/src/node/server/sourcemap.ts b/packages/vite/src/node/server/sourcemap.ts
index 0b9bcf9284754b..88cbafc344c739 100644
--- a/packages/vite/src/node/server/sourcemap.ts
+++ b/packages/vite/src/node/server/sourcemap.ts
@@ -1,9 +1,8 @@
 import path from 'path'
 import { promises as fs } from 'fs'
 import type { Logger } from '../logger'
-import { createDebugger, normalizePath } from '../utils'
+import { createDebugger } from '../utils'
 import type { SourceMap } from 'rollup'
-import { maybeVirtualHtmlSet } from '../plugins/html'
 
 const isDebug = !!process.env.DEBUG
 const debug = createDebugger('vite:sourcemap', {
@@ -43,7 +42,6 @@ export async function injectSourcesContent(
           sourcePath = path.resolve(sourceRoot, sourcePath)
         }
         return fs.readFile(sourcePath, 'utf-8').catch(() => {
-          if (maybeVirtualHtmlSet.has(normalizePath(sourcePath))) return null
           missingSources.push(sourcePath)
           return null
         })