diff --git a/examples/basic/test/sourcemap.test.ts b/examples/basic/test/sourcemap.test.ts
new file mode 100644
index 000000000000..5642f9155fb0
--- /dev/null
+++ b/examples/basic/test/sourcemap.test.ts
@@ -0,0 +1,8 @@
+/* eslint-disable @typescript-eslint/quotes */
+import { expect, it } from 'vitest'
+
+it('should have sourcemaps', () => {
+  expect('\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9').toBeTruthy()
+  expect("\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9").toBeTruthy()
+  expect(`\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9`).toBeTruthy()
+})
diff --git a/packages/vite-node/src/source-map.ts b/packages/vite-node/src/source-map.ts
index 4adccd27a2d5..e9d67c71bd05 100644
--- a/packages/vite-node/src/source-map.ts
+++ b/packages/vite-node/src/source-map.ts
@@ -41,7 +41,7 @@ export function withInlineSourcemap(result: TransformResult, options: {
   })
 
   // to reduce the payload size, we only inline vite node source map, because it's also the only one we use
-  const OTHER_SOURCE_MAP_REGEXP = new RegExp(`//# ${SOURCEMAPPING_URL}=data:application/json[^,]+base64,(.+)`, 'g')
+  const OTHER_SOURCE_MAP_REGEXP = new RegExp(`//# ${SOURCEMAPPING_URL}=data:application/json[^,]+base64,([A-Za-z0-9+/=]+)$`, 'gm')
   while (OTHER_SOURCE_MAP_REGEXP.test(code))
     code = code.replace(OTHER_SOURCE_MAP_REGEXP, '')
 
diff --git a/test/vite-node/test/sourcemap.test.ts b/test/vite-node/test/sourcemap.test.ts
new file mode 100644
index 000000000000..f8e2365ce557
--- /dev/null
+++ b/test/vite-node/test/sourcemap.test.ts
@@ -0,0 +1,92 @@
+/* eslint-disable no-template-curly-in-string */
+import type { TransformResult } from 'vite'
+import { describe, expect, it } from 'vitest'
+import type { SourceMap } from 'rollup'
+import { withInlineSourcemap } from '../../../packages/vite-node/src/source-map'
+
+it('regex match', () => {
+  const regex = /\/\/# sourceMappingURL=data:application\/json;charset=utf-8;base64,([A-Za-z0-9+/=]+)$/gm
+  expect('function foo(src) {\n  return `//# sourceMappingURL=data:application/json;base64,${src}`;\n}\nObject.defineProperty(__vite_ssr_exports__, "foo", { enumerable: true, configurable: true, get(){ return foo }});\n'.match(regex)).null
+  expect(`function foo(src) {
+    return \`//# sourceMappingURL=data:application/json;base64,\${src}\`;
+  }
+  Object.defineProperty(__vite_ssr_exports__, "foo", { enumerable: true, configurable: true, get(){ return foo }});
+  
+  //# sourceMappingSource=vite-node
+  //# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udHMifQ==`.match(regex)).deep.eq(['//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udHMifQ=='])
+  expect(`function foo(src) {
+    return \`//# sourceMappingURL=data:application/json;base64,\${src}\`;
+  }
+  Object.defineProperty(__vite_ssr_exports__, "foo", { enumerable: true, configurable: true, get(){ return foo }});
+  
+  //# sourceMappingSource=vite-node
+  //# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udHMifQ==`.replace(regex, '')).not.include('//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udHMifQ==')
+})
+
+describe('withInlineSourcemap', () => {
+  const input: TransformResult = {
+    code: 'function foo(src) {\n  return `//# sourceMappingURL=data:application/json;base64,${src}`;\n}\nObject.defineProperty(__vite_ssr_exports__, "foo", { enumerable: true, configurable: true, get(){ return foo }});\n',
+    map: {
+      version: 3,
+      mappings: 'AAAO,SAAS,IAAI,KAAqB;AACvC,SAAO,qDAAqD;AAC9D;iHAAA',
+      names: [],
+      sourceRoot: undefined,
+      sources: [
+        '/foo.ts',
+      ],
+      sourcesContent: [
+        'export function foo(src: string): string {\n  return `//# sourceMappingURL=data:application/json;base64,${src}`\n}\n',
+      ],
+      file: '/src/foo.ts',
+    } as unknown as SourceMap,
+    deps: [
+    ],
+    dynamicDeps: [
+    ],
+  }
+  const options = {
+    root: '/',
+    filepath: '/foo.ts',
+  }
+
+  it('Check that the original sourcemap in the string isnot removed', () => {
+    expect(withInlineSourcemap(input, options).code).contain('return `//# sourceMappingURL=data:application/json;base64,${src}`')
+  })
+  it('Check that the appended sourcemap is removed', () => {
+    input.code = `function foo(src) {
+      return \`//# sourceMappingURL=data:application/json;base64,\${src}\`;
+    }
+    Object.defineProperty(__vite_ssr_exports__, "foo", { enumerable: true, configurable: true, get(){ return foo }});
+    
+    //# sourceMappingSource=other
+    //# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udtest==`
+
+    expect(withInlineSourcemap(input, options).code).not.toContain('//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udtest==')
+  })
+  it('Check that the vite-node sourcemap isnot removed', () => {
+    input.code = `function foo(src) {
+      return \`//# sourceMappingURL=data:application/json;base64,\${src}\`;
+    }
+    Object.defineProperty(__vite_ssr_exports__, "foo", { enumerable: true, configurable: true, get(){ return foo }});
+    
+    //# sourceMappingSource=vite-node
+    //# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udHMifQ==`
+
+    expect(withInlineSourcemap(input, options).code).include('//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9uIjozLCJtYXBwaW5ncyI6IkFBQU8sU0FBUyxJQUFJLEtBQXFCO0FBQ3ZDLFNBQU8scURBQXFEO0FBQzlEO2lIQUFBIiwibmFtZXMiOltdLCJzb3VyY2VzIjpbIi4uL2Zvby50cyJdLCJzb3VyY2VzQ29udGVudCI6WyJleHBvcnQgZnVuY3Rpb24gZm9vKHNyYzogc3RyaW5nKTogc3RyaW5nIHtcbiAgcmV0dXJuIGAvLyMgc291cmNlTWFwcGluZ1VSTD1kYXRhOmFwcGxpY2F0aW9uL2pzb247YmFzZTY0LCR7c3JjfWBcbn1cbiJdLCJmaWxlIjoiL3NyYy9mb28udHMifQ==')
+  })
+  it('Check that the vite-node in real code', () => {
+    input.code = `
+import { expect, it } from 'vitest'
+
+it('should have sourcemaps', () => {
+  expect('\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9==').toBeTruthy()
+  expect("\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9==").toBeTruthy()
+  expect(\`\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9==\`).toBeTruthy()
+})
+    `
+    expect(withInlineSourcemap(input, options).code)
+      .include('\'\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9==\'')
+      .include('"\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9=="')
+      .include('`\n//# sourceMappingURL=data:application/json;charset=utf-8;base64,eyJ2ZXJzaW9==`')
+  })
+})