diff --git a/packages/vitest/src/runtime/entry.ts b/packages/vitest/src/runtime/entry.ts
index 11fcf3b868bf..2799b631d57a 100644
--- a/packages/vitest/src/runtime/entry.ts
+++ b/packages/vitest/src/runtime/entry.ts
@@ -7,6 +7,11 @@ import { startTests } from './run'
 export async function run(files: string[], config: ResolvedConfig): Promise<void> {
   await setupGlobalEnv(config)
 
+  const workerState = getWorkerState()
+
+  // reset mock state
+  workerState.mockMap.clear()
+
   for (const file of files) {
     const code = await fs.readFile(file, 'utf-8')
 
@@ -15,7 +20,6 @@ export async function run(files: string[], config: ResolvedConfig): Promise<void
     if (!['node', 'jsdom', 'happy-dom'].includes(env))
       throw new Error(`Unsupported environment: ${env}`)
 
-    const workerState = getWorkerState()
     workerState.filepath = file
 
     await withEnv(env as BuiltinEnvironment, config.environmentOptions || {}, async() => {